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KMID : 0388019930040040047
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Korean Journal Gynecologic Oncology and Colposcopy
1993 Volume.4 No. 4 p.47 ~ p.55
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Flow Cytometric Analysis of DNA Content in Cervical Neoplasia Patients Infected with Human Papillomavirus Types 16 and 18
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Abstract
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To investigate the role of human papillomavirus(HPV) infection on DNA infection on DNA content of cervical cancers, thirty―seven cases of cervical squamous carcinoma were studied with the method of flow cytometry for deoxyribonucleic acid(DNA)
content
and Southern blot hybridization for typing of HPV type 16 or type 18 NDA in combination with other clinical parameters.
There were 18 diploid cases(48.6%) and 19 aneuploid(51.4%). The mean age of the patients with diploid and aneuploid tumors was 51.6 years and 53.6% years, respectively. No significant age difference was found between diploid and aneuploid groups.
Six of 13(46.2%) carcinoma in situ and 13 of 24 (54.2%) invasive cervical carcinoma were aneuploid tumors was not significantly different between the two groups. Six of 10 tumors(60.0%) in stage I, 5 of 11 tumors(45.5%) in stageⅡ, 2 of 3
tumors(66.7%)
in stage Ⅲ were aneuploid. The frequency was not significantly different according to the clinical stage.
Aneuploidy was present in 50.0% of HPV 16 positive lesion, and 41.7% of PHV 16 and 18 negative lesion. There was no statistically difference in the incidence of aneuploidy when all HPV lesion were compared.
Of 25 patients with PHV 16 or 18 positive lesion, aneuploidy was demonstrated in the specimen of four of eight patients with invasive cervical carcinoma.
In summary, we found that the incidence of DNA aneuploidy was somewhat higher when HPV 18 positive lesion was compared to PHV 16 and 18 negative lesion but the difference was not statistically significant.
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KEYWORD
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